PARP1 antibody - 100 µg
Clonality: Monoclonal
Clone: 4A9
Isotype: IgG1
Immunogen: poly (ADP-ribose) polymerase 1
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 115 kDa, 89 kDa
Uniprot: P09874
Gene id: 142
Background: PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis.
Field of research: Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IF
Dilution: WB: 1:5000 - 1:30000; IHC: 1:100 - 1:1000; IF: 1:50 - 1:500
Target: PARP1
Purification: protein A+G purified
Reactivity: Human, Mouse, Rat
The Universal Fit-Neptune 0.5–10 µL XL pipette tips are extra-long tips made from pure polypropylene for accurate handling of very small liquid volumes. They are built to fit most common pipette brands and deliver precise transfer between 0.5 and 10 microliters. The XL length allows access to deep or narrow tubes without contamination from contact with the tube walls. The inner surface is very smooth to prevent liquid from sticking inside the tip, which helps reduce sample loss. These tips are available with or without aerosol barriers and come in sterile or non-sterile formats. Sterile tips are certified free of RNase, DNase, pyrogens, and ATP. They can be autoclaved without melting or warping. The tips come in rack, bulk, or reload packaging and are designed for consistent fit and secure sealing on most pipettes.