Innate and adaptive immune responses in HCV infections 

Markus H. Heim1,2,⇑ , Robert Thimme3,⇑ 1 Division of Gastroenterology and Hepatology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland; 2 Department of Biomedicine, University of Basel, Hebelstrasse 20, 4031 Basel, Switzerland; 3 Department of Medicine, Clinic for Gastroenterology, Hepatology, Endocrinology, Infectious Diseases, University Hospital Freiburg, Freiburg, Germany 

cat name price
GSB-1L SDS-Blue™ (SDS-PAGE protein stain, ready to use) - 1 L € 150
0004-5540115 TC Tubes Round PP - 15mL € 0
0004-rHuGM-CSF-300 Recombinant Human GM-CSF - 300 ug € 385
0043-2110.B 1000 uL Tips in Bags € 90
0043-BT1000 Neptune Barrier Tips - 1000uL // Universal Fit  // 8 racks of 96/pk tips each € 72
0043-BT10XL Neptune Barrier Tips - 10 µL // Extended Length Filter Tips // 10 racks of 96/pk tips each € 72
0043-BT200 Neptune Barrier Tips - 200uL // Universal Fit // 10 racks of 96/pk tips each € 72
0043-BT1250 Neptune Barrier Tips - 1250uL // Universal Fit // 8 racks of 96/pk tips each € 72
0043-BT10 Neptune Barrier Tips - 10uL // Universal Fit // 10 racks of 96/pk tips each € 72
0043-BT100 Neptune Barrier Tips - 100 µL // Filter Tips // 10 racks of 96/pk tips each € 72
0841-CATB0871 Capilia™ TB Neo - 10 Tests € 64
0948-PS002-L05X 5X Concentrate COOMASSIE nano Protein Staining Solution -1000 ml € 108
0004-GMPhuIL4-50UG GMP Recombinant Human Interleukin-4 (IL-4), 50 ug € 483
0173-RB2103DD Rapid Canine Brucella Ab Kit - 10 Tests € 117
0841-CAHM1670 Capilia™ hMPV - 20 Tests € 150
0173-RG1801DD Rapid Rabies Ag Test Kit - 10 Tests € 117
0347-9621 8-Port Manifolds Sterile, Indiv. Wrapped - 10 Manifolds/Unit € 98
0194-ISB1L InstantBlue - 1L € 160
0094-4583-01 Tissue-Tek® OCT Compound - 125mL € 100
0004-GSB1 SDS-Blue™ Coomassie blue-based protein staining solution (Ready-To-Use) - 1 Liter € 150
0043-BT20 Neptune Barrier Tips - 20uL // Universal Fit // 10 racks of 96/pk tips each € 72
0043-2342S3.SU Neptune Barrier Tips - 0.5-10uL XL // Universal Fit Extended Length Pre-Sterile // 10 racks of 96/pk tips each € 120
0580-JT90-R001S T-Pro Aqua EZ Clean (s) 100ml € 90
0094-4566 Tissue-Tek Cryomold Intermediate 15x15x5mm - 100 Units € 167
0173-AXS-1114683-250ML (AXS-1114683) Polymorphprep - 250mL € 68
0388-302-C DirectPCR Lysis Reagent (Cell) - 100mL € 300
0877-1856 (AXS-1114544) Lymphoprep - 250ml € 62
0751-A138 Factor I Purified Human Complement Protein € 611
0841-CARS0970 Capilia™ RSV Neo - 20 Tests € 150
0004-RHIL2-08F02 rHu IL2, 3MIU € 249
0043-BT300 Neptune Barrier Tips - 300uL // Universal Fit // 10 racks of 96/pk tips each € 72
0644-PCT02 Plant Preservative Mixture (PPM) - 100mL € 100
0813-GR501-01 Ultra GelRed (10.000X) - 0.5mL € 110
0388-101-T DirectPCR Lysis Reagent (Mouse Tail) - 50 mL € 140
0388-102-T DirectPCR Lysis Reagent (Mouse Tail) - 100 mL € 220
0841-CAAD0370 Capilia™ Adeno Neo - 20 Tests € 150
0094-4565 Tissue-Tek Cryomold Intermediate 10x10x5mm - 100 Units € 167
0323-WAK-ZF-1 Biocidal ZF 1 x 1 Liter Spray € 95
0841-CATB0877 Capilia™ TB Neo Extraction buffer - 20mL/Bottle € 60
0877-1893 (AXS-1114542) OptiPrep™ Density Gradient Media - 250mL € 165
0065-DIUR-100 QuantiChrom™ Urea Assay Kit - 100 assays € 176
0171-G931 qPCR AAV Titer Kit - 100 Reactions € 230
0644-PCT01 Plant Preservative Mixture (PPM) - 30mL € 40
0323-WAK-AQ-250-50L AquaClean - 250 mL concentrate € 110
0171-LV900 qPCR Lentivirus Titration kit - 100 reactions € 230
0644-PCT03 Plant Preservative Mixture (PPM) - 250mL € 230
  Avian Influenza Virus (AIV) Antigen Rapid Test Card - 25T € 180
0316-MTF500 Michel's Transport Fluid - 500ml € 85
0316-MTW500 Michel's Transport Wash Buffer 500ML € 85
22/01/6164 Chicken Embryo Extract - Frozen, 20ml € 250
  SDS-Blue™ (SDS-PAGE protein stain, ready to use) - 500mL € 80
0094-4557 Tissue-Tek Cryomold Intermediate 25x20x5mm - 100 Units € 167
0813-C115-01 ClonExpress Ultra One Step Cloning Kit - 25 Reactions € 268
0813-P505-d1 Phanta Max Super-Fidelity DNA Polymerase - 100U € 127
0813-P510-01 2X Phanta Flash Master Mix - 1mL € 114
0813-P515-01 2X Phanta Max Master Mix - 1mL € 91
0813-P520-01 2X Phanta Flash Master Mix (Dye Plus) - 1 mL € 114
0813-Q712-02 Taq Pro Universal SYBR qPCR Master Mix - 500 Reactions (20 µL/Reaction) € 322
0813-R323-01 HiScript III RT SuperMix for qPCR (+gDNA wiper) - 100 Reactions (20µL/Reaction) € 435
0841-CAFL0571 Capilia™ Flu Neo - 10 Tests € 96
0954-4AME3 Anti-HAV IgM Qualitative ELISA kit (Sensitivity: 61.7 U/ml) - 96 wells plate € 564
1007-314036 /313016? 1000 μl Filter universal Pipette Tips, Filter, Racked, Sterile, 96/pk - 10 Racks € 32
0019-DENZ-500 Gentodenz - 500G € 320
0037-41003 GelRed Nucleic Acid Stain 10,000X in water - 0.5 ml € 200
0065-DIHB-250 QuantiChrom Hemoglobin Assay Kit - 250 tests € 327
0527-PK401 FD Rapid GolgiStain™ Kit (Large, for up to 50 mouse brains) € 1,423
0644-PCT04 Plant Preservative Mixture (PPM) - 500ml € 380
0173-GEN15-01 Rapid AIV Ag Test Kit - 30 Tests/Kit € 254
0173-RC1112DC Rapid CPV/CCV/Giardia Ag - 5 Tests € 73
0173-RG1101DD CPV Ag Rapid Test Kit - 10 Tests € 212
0235-LM-S2041/1000 Dulbecco's PBS (1x), without Ca & Mg, without Phenol Red (Phosphate-buffered Saline, PBS) - 1000 mL € 30
0323-WAK-AQA-250-50L Acryl AquaClean 250 ml concentrate € 150
0841-CAST1170 Capilia™ Strep A - 20 Tests € 168
0877-1858 (AXS-1115758) Lymphoprep - 500ml € 74
0001-NAT1330 [HS-200] Histoclear I - 1 US Gallon (3,7L) € 320
0065-POMG-25H Malachite Green Phosphate Assay Kit - 2500 tests € 234
0527-PK401A FD Rapid GolgiStain™ Kit (Small, for up to 25 mouse brains) € 920
0644-PCT05 Plant Preservative Mixture (PPM) - 1000mL € 700
0173-RB2151DD CPV Ab Rapid Test Kit - 10 Tests € 212
0444-INT-03 ASSURANCE RF Interference Test Kit, Five separate samples, at different concentrations - 5 vials € 1,935
0445-7009 Complete Freund's Adjuvant, 2mg/ml M. Tuberculosis H-37 RA € 90
0841-CATB0870 Capilia™ TB Neo - 100 Tests € 338
0001-NAT1334 [HS-202] HistoClear II - 1 US Gallon - 3,7 L € 350
0001-NW-300 Nuc-Wipes PK100 € 65
0544-MBS2508822-96T FOXO3 elisa kit :: Human FOXO3 ELISA Kit - 96 Tests € 830
0544-MBS9318876-96T GLI2 elisa kit :: Human GLI family zinc finger 2 ELISA Kit - 96-Strip-Wells € 1,248
0767-GRS-20059 Bird Flu H5 Antigen Rapid Test Card - 25Tests/Kit € 180
0173-GEN15-05 Rapid H5 AIV Ag Test Kit, 30 tests/kit € 410
0173-RB2152DD CDV Ab Rapid test kit - 10 Tests € 125
0173-RB2301DD Rapid Bovine Brucella Ab Kit - 10 Tests € 80
0173-RG1102DD Rapid HW Ag 2.0 - 10 Tests € 120
0173-RG1104DD Rapid CCV Ag Test Kit - 1Test x 10/Kit € 95
0214-F410 E. coli HCP ELISA Kit - 96 Wells € 1,200
0388-301-C DirectPCR Lysis Reagent (Cell) - 50 mL € 192
0399-CSB-E14020h-96T Human Soluble Interleukin-7 Receptor (sIL-7R) ELISA Kit - 96 wells plate € 1,139
0841-CAMP1571 Capilia™ Mycoplasma - 10 Tests € 96
0841-CARS0971 Capilia™ RSV Neo - 10 Tests € 72
0877-18003 (AXS-1002424) Nycodenz - 500 g € 350
0903-DA1431 Detection Kit for Monkeypox Virus DNA (PCR Fluorescence Probing)  - 48 Tests/Kits € 500
0987-VVD20 C. Brucella Rapid Antibody Test Kit - S/P/WB - 10 Tests € 123
0001-EC-890-1L ProtoGel (30%) - 1 liter € 300
0001-NAT1236-50g Dextran Sulfate 50G € 245
0001-NAT1336 Histo-Clear II Histology Clearing Agent - 5 US Gallons € 950
0173-7D2741-25T Determine TB LAM Ag - 25 tests € 280
0494-01-0004 Rabbit anti-EBOV VLP antiserum - 250 uL € 635
0501-Exo-CH13 HQExo™ Exosome-SK-N-SH - 1 vial/(> 1x10^8 particles) € 1,970
0501-Exo-CH14 QExo™ Exosome-SK-N-SH - 1 vial/(> 1x10^8 particles € 1,970
0501-IL10RB-4431H-300 UG Recombinant Human IL10RB Protein, His (Fc)-Avi-tagged, 20-220 a.a., Biotinylated, 300 ug € 9,635
0511-MCDB 131–Powder, 50LT MBP02 w/o glucose, L-glutamine, phenol red and sodium bicarbonate - Powder, 50LT € 135
0511-PPL33 Plant Preservative Mixture (PPM) - 10 mL € 20
0527-PF101 Phosphate-Buffered 4% Paraformaldehyde, 1000 ml € 186
0527-PK301 FD NeuroSilver™ Kit II (Large), for 300 sections € 1,259
0544-MBS2024205-96T EFNB2 elisa kit :: Human Ephrin B2 (EFNB2) ELISA Kit - 96 Wells € 1,075
0563-CTK-R0024C HCV Ab Plus Combo Rapid Test, 30 tests/kit € 133
0568-NC1286079 Exosome Isolation Kit for Cell Culture Media (10rxn) € 1
0639-abx082155 Bind-Silane - 25mL € 240
0644-BAP100 Benzylaminopurine (BAP) Solution 1mg/1ml, 1.00 LBS € 40
0751-Rb113 C3 Protein (Rabbit) - 100 µg/vial € 420
0763-EP-CL-0005 293T [HEK-293T] Cell Line - 1×10⁶ cells/vial € 878
0813-MD104-02 100 bp DNA Ladder - 100 reactions € 58
0813-P112-02 2X Taq Master Mix (Dye Plus) - 15×1mL € 197
0813-P222-03 2X Rapid Taq Master Mix - 50×1mL (2000 Reactions) € 860
0173-RB2201DD Rapid FIV Ab - 10 Tests € 150
0173-RC1204DD Rapid FIV Ab/FeLV Ag - 10 Tests € 150
0173-RG1201DD Rapid FeLV Ag - 10 Tests € 150
0337- X2555-10 Cleanascite™ Lipid Removal Reagent and Clarification, 10 ml € 546
0388-401-E DirectPCR Lysis Reagent (Mouse Ear) - 25 mL € 192
0388-402-E DirectPCR Lysis Reagent (Ear) - 50 mL € 300
0813-Q712-03 Taq Pro Universal SYBR qPCR Master Mix - 2500 Reactions (20 µL/Reaction) € 1,450
0870-ABL-TC0757 CYNOM-K1, Monkey Cynomologus skin cell - 1 vial € 3,524
0947-UC0104 EasyNAT Nucleic Acid Amplification and Detection Analyzer € 10,000


PATHOGEN CO-INFECTION


HIV-1, Tubercolosis, Malaria

and Hepatitis C virus


 agenda
 participants
 PathCo Newsletter
 PathCo Brochure




Pathogen Co-infection: HIV-1, Tuberculosis, Malaria and hepatitis C virus


  • Acquired immune deficiency syndrome (AIDS), tuberculosis (TB) and malaria are the primary infectious diseases causing death world wide. TB is responsible for a third to a half of human immunodeficiency virus type 1 (HIV-1)-associated deaths, particularly in sub Saharan Africa and South East Asia. Malaria is widely spread in tropical Africa and accounts for approximately 800,000 deaths every year. In addition to these pathogens, 170 million people are infected with hepatitis C virus (HCV), which may lead to chronic liver disease, liver cirrhosis and hepatocellular carcinoma. Because of shared routes of transmission, HCV co-infection is recognized as a major cause of morbidity and mortality among HIV-1 infected persons.


  • Effective drugs to control these infectious agents are limited and treatment is made even more challenging by the development of drug-resistant pathogens. Importantly, co-infection with one or more of these pathogens limits the efficacy of available drugs. The epidemiological and clinical features of co-infected subjects is well documented, however, there is a paucity of basic scientific studies addressing the molecular interaction(s) between these pathogens. Variant pathogen strains are known to influence infection and disease outcome, however, the underlying mechanism(s) are unknown.


  • PathCo consortium propose that pathogen evasion and dysregulation of host immune responses play a key role in co-infection associated morbidity. We will test this hypothesis by developing in vitro and ex vivo co-infection model systems to study pathogen interactions. The specific objectives of this project are to improve our understanding of pathogen co-infection effect(s) on host innate and adaptive immune responses and to develop new approaches to dissect pathogen interactions: ranging from the genesis of fluorescent labelled viruses to state-of-the-art tissue explant culture systems and novel humanised mouse models. Translational studies of infected subjects will define pathogen-specific effects on host immune responses and consequences for disease progression. It is imperative that such interactions are elucidated before evaluating new prophylactic or therapeutic strategies in co-infected individuals.



PathCo Project Focus

  • The PathCo project brings together powerful multidisciplinary technologies that will improve our understanding of the complex interactions between infectious agents and the host immune response that will significantly improve the management of co-infection associated disease.

 

  • Recent developments in each of the disease disciplines enable the design of model systems that support pathogen co-infection, highlighting the timeliness of PathCo’s mission to study the biological and immunological consequences of co-infection(s).


  • Ultimately such knowledge will be instrumental in the rational design of new therapies and vaccines to control HIV-1, TB, malaria and HCV. In parallel to generating and utilizing new tools to understand the basic biology of pathogen co-interactions the PathCo consortium members will develop novel anti-microbials for translational research. 

      The PathCo project will identify new targets for therapy and vaccine design, whilst validating known agents for their ability to block pathogen transmission and to target antigen        or anti-pathogen components to dendritic cells (DCs).


  • The PathCo consortium consists of ten beneficiaries from five EU countries (Netherlands, UK, France, Germany and Italy) and one beneficiary from non-EU countries (South Africa).


  • PathCo team is a well-balanced team of immunologists, virologists, clinicians, statisticians, epidemiologists with expertise in HIV/AIDS, TB, malaria and hepatitis C infection research and has the most appropriate scientific and technical background as well as the animal models and instrumentation required to fulfill the goals of this project and to succeed in its mission. PathCo beneficiaries will have access to well-characterised established cohorts of co-infected patients from different geographical locations. Given the widespread global nature of the pathogens under study it is imperative that we are not biased in our selection of patients.





virus



Project info

Acronym: PathCo

EC Contribution: € 5.909.690

Duration: 60 months

Starting date: 01/11/2012

Instrument: Collaborative project


Coordinator:

Bill Paxton

The University of Liverpool Department of Clinical Infection, Microbiology and Immunology Institute of Infection and Global Health

The Ronald Ross Building West Derby Street 8 Liverpool L69 7BE United Kingdom





This project is supported through Coordination Theme 1 (Health) of the European Community's FP7. Grant agreement number

HEALTH-F3-2012-305578


Last update: 07/12/2017



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We build modular solutions.

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PathCo is split into 5 basic research areas encompassing

the following objectives


-> To define the genetic and molecular basis underlying

TB regulation of HIV-1 replication

-> To define the role of HIV-1 infection and associated

inflammation on HCV replication

-> To identify common pathways defining Malaria

and HCV liver tropism

-> To generate humanized mouse model systems

that support pathogen co-infections

-> To identify the effect(s) of HIV-1 infection on HCV

specific host immune responses



The PathCo project brings together powerful multidisciplinary technologies that will improve our

understanding of the complex interactions between infectious agents and the host immune response that will significantly improve the management of co-infection associated disease.


 Recent developments in each of the disease disciplines enable the design of model systems that support pathogen co-infection, highlighting the timeliness of PathCo’s mission to study

the biological and immunological consequences of co-infection(s). Ultimately such knowledge will be instrumental in the rational design of new therapies and vaccines to control HIV-1, TB, malaria and HCV.

 In parallel to generating and utilizing new tools to understand the basic biology of pathogen co-interactions the PathCo consortium members will develop novel anti-microbials for translational research. 


The PathCo project will identify new targets for therapy and vaccine design, whilst validating known agents for their ability to block pathogen transmission and to target antigen or anti-pathogen components to dendritic cells (DCs).


PATHOGEN CO-INFECTION - THE PROBLEM

Acquired immune deficiency (AIDS), tuberculosis (TB) and malaria are the primary infectious diseases causing death worldwide. TB is responsible for a third to a half of human immunodeficiency virus type 1 (HIV-1)-associated deaths, particularly in sub Saharan Africa and South East Asia. Malaria is widely spread in tropical Africa and accounts for approximately 800,000 deaths every year. In addition to these pathogens, 170 million people are infected with hepatitis C virus (HCV), which leads to chronic liver disease. Because of shared routes of transmission, HCV co-infection is recognized as a major cause of morbidity and mortality among

HIV-1 infected persons.

The epidemiology and clinical features of co-infected subjects is well documented, however, there is a paucity of basic scientific studies addressing the interactions between these pathogens. There is undoubtedly a complex interplay between pathogens and the host

immune response.


PATHCO FOCUS AND OUTCOME

PathCo consortium proposes that pathogen evasion and dysregulation of host immune responses play a key role in co-infection associated morbidity. Within this Project will test this hypothesis by developing in vitro and ex vivo co-infection model systems to study co-pathogen interactions. The specific objectives of PathCo are to improve our understanding of pathogen

co-infection effect(s) on host innate and adaptive immune responses and to develop new approaches to dissect pathogen interactions: ranging from the genesis of fluorescent labelled viruses to state-of-the-art tissue explant culture systems and novel humanised mouse

models. Translational studies of infected subjects will define pathogen-specific effects on host immune responses and consequences for disease progression.

It is imperative that such interactions are elucidated before evaluating new prophylactic or therapeutic strategies in co-infected individuals.

PATHCO ALLIANCE

The PathCo consortium consists of nine beneficiaries from four EU different countries (UK, France, Germany and Italy) and one beneficiary from non-EU countries (South Africa).

PathCo is a well-balanced team of immunologists, virologists, clinicians, statisticians, epidemiologists with expertise in HIV/AIDS, TB, malaria and hepatitis C infection research and has the most appropriate scientific and technical background as well as the animal models and instrumentation required to fulfill the goals of this project and to succeed in its mission.

PathCo beneficiaries will have access to well-characterised established cohorts of co-infected patients from different geographical locations.


Technology

In vitro

systems

Advanced

Animal

Models

Human

Immunology

HIV

TB

HIV

HCV

HIV

Malaria

TB

regulation of

HIV replication

TB

regulation of

HIV replication

HIV infectioninflammation

in HCV

replication

Generation

humanized

mouse models

for mono- and

co-infection

HIV infection

on HCV

specific host

immune

responses

Common

pathways

of Malaria and

HCV entry into

hepatocytes

infection

Generation

humanized

mouse models

for mono- and

co-infection



PROJECT ORGANIZATION


pathogen Co-infection:

HIV-1, Tuberculosis, Malaria and hepatitis C virus

University of Birmingham

Jane McKeating - Gurdyal Besra

United Kingdom

www.birmingham.ac.uk

institUt nationaL de La santé

et de La reCherChe médiCaLe

Olivier Silvie - France

www.inserm.fr

UniversitaetskLinikUm freiBUrg

Robert Thimme - Germany

www.uniklinik-freiburg.de

University of oxford

Tao Dong - United Kingdom

www.ox.ac.uk

imperiaL CoLLege of sCienCe,

teChnoLogy and mediCine

Carolina Herrera - Xiao-Ning Xu

United Kingdom- www3.imperial.ac.uk

University of Cape toWn

Robert Wilkinson - South Africa

www.uct.ac.za

institut pasteur

James Di Santo - France

www.pasteur.fr

aLta riCerCa e sviLUppo

in BioteCnoLogie s.r.L.U.

Riccardo Bertini - Italy

www.altaweb.eu

PATHCO PARTNERS

pathogen Co-infeCtion:

HIV-1, Tuberculosis, Malaria

and hepatitis C virus


Coordinator:

Prof William a paxton phd diC

Department of Clinical Infection,

Microbiology and Immunology

Institute of Infection and Global Health

University of Liverpool

216e, Ronald Ross Building

8 West Derby Street

Liverpool L69 7BE

w.a.paxton@liverpool.ac.uk

www.pathco.org

EC Contribution: € 5.909.690

Duration: 60 months

Starting date: 01/11/2012

University of LiverpooL

Bill Paxton - United Kingdom

www.liv.ac.uk


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