NPC1 antibody - 100 µg
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: Niemann-Pick disease, type C1
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 160 kDa
Uniprot: O15118
Gene id: 4864
Background: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal
lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo'(TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome(LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes
lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes
lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. (Microbial infection) Acts as an endosomal entry receptor for ebolavirus.
Field of research: Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC
Dilution: WB: 1:500-1:2000; IHC: 1:20-1:200
Target: NPC1
Purification: Immunogen affinity purified
Reactivity: Human, Mouse
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