OGA antibody - 100 µg
Clonality: Monoclonal
Clone: 9B9
Isotype: IgG2a
Immunogen: meningioma expressed antigen 5(hyaluronidase)
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 130 kDa
Uniprot: O60502
Gene id: 10724
Background: Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro)(PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity(PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro), but has about six times lower specific activity than isoform 1.
Field of research: Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IF, IHC
Dilution: WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Target: OGA
Purification: Protein A+G purification
Reactivity: Human
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