PCMV DR8.91 plasmid 2 ug
PCMV-DR8.91 plasmid (2 µg) is a third-generation lentiviral packaging construct derived from HIV-1, designed for high-efficiency pseudotyped viral particle production in transient transfection systems. It encodes the essential gag, pol, tat, and rev genes under the control of the human CMV immediate-early promoter (PCMV), ensuring robust transcription in mammalian cells, particularly HEK293T. This plasmid excludes accessory HIV-1 genes (vif, vpr, vpu, nef) to enhance biosafety, making it replication-incompetent in target cells. DR8.91 provides structural and enzymatic components required for lentiviral assembly, reverse transcription, and integration but does not contain the viral packaging signal (Ψ), which is instead supplied by a co-transfected transfer vector. Typically used with envelope plasmids such as VSV-G (e.g., pMD2.G), PCMV-DR8.91 supports the generation of high-titer, self-inactivating lentiviral vectors for stable transgene delivery in dividing and non-dividing mammalian cells.
The Universal Fit-Neptune 0.5–10 µL XL pipette tips are extra-long tips made from pure polypropylene for accurate handling of very small liquid volumes. They are built to fit most common pipette brands and deliver precise transfer between 0.5 and 10 microliters. The XL length allows access to deep or narrow tubes without contamination from contact with the tube walls. The inner surface is very smooth to prevent liquid from sticking inside the tip, which helps reduce sample loss. These tips are available with or without aerosol barriers and come in sterile or non-sterile formats. Sterile tips are certified free of RNase, DNase, pyrogens, and ATP. They can be autoclaved without melting or warping. The tips come in rack, bulk, or reload packaging and are designed for consistent fit and secure sealing on most pipettes.
