PBBR1MCS2 TAC EGFP plasmid 2ug
pBBR1MCS-2-TAC-EGFP Plasmid (2 µg) is a broad-host-range, low-copy-number, mobilizable plasmid engineered with a pBBR1 origin of replication, enabling replication across a diverse array of Gram-negative bacterial species, including Escherichia coli, Pseudomonas spp., and Agrobacterium tumefaciens. The plasmid carries a multiple cloning site (MCS) within the lacZα gene for blue/white screening and confers kanamycin resistance (Kan^R) for antibiotic selection. Downstream of the MCS, the tac promoter, a hybrid of the trp and lac promoters, drives constitutive or IPTG-inducible high-level expression of the enhanced green fluorescent protein (EGFP) gene, allowing for real-time, non-invasive fluorescence-based monitoring of gene expression and plasmid maintenance. The EGFP open reading frame is codon-optimized for bacterial expression and lacks any eukaryotic introns. This plasmid is ideal for fluorescence reporter assays, microbial tracking, and heterologous protein expression studies in environmental or synthetic biology contexts.
The Universal Fit-Neptune 0.5–10 µL XL pipette tips are extra-long tips made from pure polypropylene for accurate handling of very small liquid volumes. They are built to fit most common pipette brands and deliver precise transfer between 0.5 and 10 microliters. The XL length allows access to deep or narrow tubes without contamination from contact with the tube walls. The inner surface is very smooth to prevent liquid from sticking inside the tip, which helps reduce sample loss. These tips are available with or without aerosol barriers and come in sterile or non-sterile formats. Sterile tips are certified free of RNase, DNase, pyrogens, and ATP. They can be autoclaved without melting or warping. The tips come in rack, bulk, or reload packaging and are designed for consistent fit and secure sealing on most pipettes.
